We propose here the quantification of the phosphorus content in cola drinks by molybdenum blue method. Molybdenum blue method was carried out in the wells of a microplate, where method miniaturization represents reduction in reagents required, improved safety, reduced waste stream, and increased sample throughput. The phosphate concentration was determinate using scanned images of the microplate. Extraction of RGB values was carried out using ImageJ, the pugling Readplate was used allowing extraction of RGB values of all wells simultaneously. A Microsoft Excel spreadsheet is afforded in Google Drive, it organize data taken from ImageJ to afford calibrations curves and phosphate concentration in cola drinks. Calibration curves were prepared at 500-2500 µg L-1 of P with good linearity (R > 0.95). Phosphate concentrations in cola drinks determinate using digital images method are statistically equivalent to concentrations determined using a spectroscopic method at a 95% confidence interval.

This review considers some of the difficulties encountered with the analysis of basic solutes using reversed-phase chromatography, such as detrimental interaction with stationary phase silanol groups. Methods of overcoming these problems in reversed-phase separations, by judicious selection of the stationary phase and mobile phase conditions, are discussed. Developments to improve the chemical and thermal stability of stationary phases are also reviewed. It is shown that substantial progress has been made in the manufacturing of stationary phases, enabling their use over a wide variety of experimental conditions. In addition, general measures to significantly extend their lifespan are discussed.

High-temperature liquid chromatography (HTLC) is a technique that presents a series of advantages in liquid phase separations, such as: reduced analysis time, reduced pressure drop, reduced asymmetry factors, modified retentions, controlled selectivities, better efficiencies and improved detectivities, as well as permitting green chromatography. The practical limitations that relate to instrumentation and to stationary phase instability are being resolved and this technique is now ready to be applied for routine determinations.